Specifications, Uses, SDS of Gentian Violet Manufacturers

	King of Chemicals
Specifications, Properties, Uses, SDS of Gentian Violet Crystal Violet; Basic Violet 3; Methylrosanilinium Chloride USP Grade Manufacturer Supplier Exporter Wholesale & Small Packs, CAS Number 548-62-9.
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Muby Chem Pvt. Ltd. is a several decades old group of companies, engaged in manufacturing, supplying, distributing, wholesale supplies of Gentian Violet Crystal Violet; Basic Violet 3; Methylrosanilinium Chloride for actual users, including retail or small pack supplies for research and development work.

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Gentian Violet Crystal Violet Manufacturer Supplier Exporter

Gentian Violet Crystal Violet; Basic Violet 3; Methylrosanilinium Chloride CAS Number 548-62-9

The Properties, Specifications and Uses of Gentian Violet Crystal Violet; Basic Violet 3; Methylrosanilinium Chloride:

CAS Number 548-62-9, EINECS EC Number 208-953-6, HS Code 320413**, Molecular Weight 407.98, Chemical Formula C25H30ClN3

Crystal violet or Gentian violet, also known as Methyl violet 10B or Hexamethyl pararosaniline chloride, is a triarylmethane dye used as a histological stain and in Gram's method of classifying bacteria. Crystal violet has antibacterial, antifungal, and anthelmintic properties and was formerly important as a topical antiseptic. Gentian violet is an antiseptic dye used to treat fungal infections of the skin. It also has weak antibacterial effects and may be used on minor cuts and scrapes to prevent infection. Crystal violet is not only used as a textile dye, but also it is used to dye paper and as a component of navy blue and black inks for printing, ball-point pens, and inkjet printers. It is also used to colourize diverse products such as fertilizers, antifreezes, detergents, and leather. Because of its antimicrobial activity, it is used to treat ich in fish.

Specifications of Gentian Violet USP Grade Crystal Violet
C25H30ClN3 407.98
Methanaminium, N-[4-[bis[4-(dimethylamino)phenyl]methylene]-2,5-cyclohexadien-1-ylidene]-Nmethyl-,chloride.
C. I. Basic violet 3.
[4-[Bis[p-(dimethylamino)phenyl]methylene]-2,5-cyclohexadien-1-ylidene]dimethylammonium chloride CAS 548-62-9.

Gentian Violet contains not less than 96.0 percent and not more than 100.5 percent of gentian violet (C25H30ClN3), calculated on the anhydrous basis.

Identification:
A: Sprinkle about 1 mg on 1 mL of sulfuric acid: it dissolves in the acid with an orange or brown red color. When this solution is diluted cautiously with water, the color changes to brown, then to green, and finally to blue.
B: Dissolve about 20 mg in 10 mL of water, and add 5 drops of hydrochloric acid. To 5 mL of this solution add tannic acid TS drop wise: a deep blue precipitate is formed.
C: To the remainder of the solution prepared for Identification test B add about 500 mg of zinc dust, and warm the mixture: rapid de-colorization occurs. Place a drop of the decolorized solution adjacent to a drop of 6 N ammonium hydroxide on a filter paper: a blue color is produced at the zone of contact.
Water: not more than 7.5%.
Residue on ignition: not more than 1.5%.
Alcohol-insoluble substances: Boil 1.0 g, accurately weighed, with 50 mL of alcohol under a reflux condenser for 15 minutes, filter through a tarred filtering crucible, wash the residue on the filter with hot alcohol until the last washing is not colored violet, and dry the crucible at 105 for 1 hour: not more than 1.0% of insoluble residue remains.
Arsenic: The limit is 0.001%.
Lead: The limit is 0.003%.
Zinc:
Zinc standard stock solution: Transfer about 1 g of zinc, accurately weighed, to a 1000-mL volumetric flask, add 50 mL of nitric acid, and mix to dissolve. Dilute with water to volume, and mix.
Standard preparation: Dilute the Zinc standard stock solution with water to obtain a Standard preparation containing 0.50 Ag of zinc per mL.
Test preparation: Weigh accurately 0.50 g of Gentian Violet in a suitable tarred crucible. Place in a low-temperature plasma ashing apparatus, and ash until a constant weight is attained. Pipette 10 mL of 6 N nitric acid into the crucible, and heat to dissolve the ash. Transfer the solution to a 500-mL volumetric flask, dilute with water to volume, and mix. Prepare a reagent blank.
Procedure: Concomitantly determine the absorbance of the Standard preparation, the Test preparation, and the reagent blank at the zinc emission line at 213.9 nm, with a suitable atomic absorption spectrophotometer (see Spectrophotometry and Light-Scattering 851 ) equipped with a zinc lamp and an air–acetylene flame, using water as the blank. The absorbance of the Test preparation, corrected for that of the reagent blank, is not greater than the absorbance of the Standard preparation, similarly corrected (0.05%).
Chromatographic purity: Dissolve 10 mg in 10 mL of methanol to obtain the Test solution. Transfer 1.0 mL of Test solution to a 100-mL volumetric flask, dilute with methanol to volume, and mix (Diluted test solution). Apply 5 AL each of the Test solution and the Diluted test solution to a suitable thin-layer chromatographic plate (see Chromatography 621 ) coated with a 0.25-mm layer of octadecylsilanized chromatographic silica gel. Allow the spots to dry, and develop the chromatogram in a suitable chromatographic chamber with a solvent system consisting of the upper layer separated from a well-shaken mixture of water, butyl alcohol, and glacial acetic acid (100:80:20), until the solvent front has moved about three-fourths of the length of the plate. Remove the plate from the chamber, allow the solvent to evaporate, and visually locate the spots on the plate: the Test solution exhibits a principal spot and not more than one secondary spot which, if present in the chromatogram from the Test solution, is not more intense than the principal spot obtained from the Diluted test solution (1.0%).
Assay: Transfer about 400 mg of Gentian Violet, accurately weighed, to a 300-mL conical flask, add 25 mL of water and 10 mL of hydrochloric acid, displace the air in the flask with carbon dioxide, and pass a stream of carbon dioxide through the flask during the assay. Add 50.0 mL of 0.1 N titanium trichloride, heat to boiling, and boil gently for 10 minutes, swirling the liquid occasionally. Cool the solution, add 5 mL of ammonium thiocyanate solution (1 in 10), and titrate with 0.1 N ferric ammonium sulfate VS until a faint red color is produced. Perform a blank determination (see Residual Titrations 541 ). Each mL of 0.1 N titanium trichloride is equivalent to 20.40 mg of C25H30ClN3.

Specifications of Methylrosanilinium Chloride BP Ph Eur Grade:
C25H30ClN3 --- 408.0 --- CAS 548-62-9
Action and use: Antiseptic dye (gentian violet).

DEFINITION
N-[4-[Bis[4-(dimethylamino)phenyl]methylene]cyclohexa-2,5-dienylidene]-N-methylmethanaminium chloride (hexamethyl-p-rosanilinium chloride). It contains not more than 10 per cent of pentamethyl-p-rosanilinium chloride and is also known as crystal violet and gentian violet.
Content: 95.0 per cent to 103.0 per cent (anhydrous substance).

CHARACTERS
Appearance: Dark green, shiny powder, hygroscopic.
Solubility: Sparingly soluble in water, freely soluble in ethanol (96 per cent) and in methylene chloride..

IDENTIFICATION
First identification: A.
Second identification: B, C
A. Infrared absorption spectrophotometry.
B. Thin-layer chromatography. To pass the test.
C. Dissolve 50 mg in water and dilute to 5 mL with the same solvent; add 3 mL of dilute sulfuric acid, 1g of zinc powder and heat gently. The mixture decolourises. Filter. To 3 mL of the filtrate add 0.5 mL of silver nitrate solution. A white turbidity is produced which slowly forms a dark, coagulating precipitate.

The MSDS-SDS Hazard Statement of Gentian Violet Crystal Violet; Basic Violet 3; Methylrosanilinium Chloride:

Gentian Violet SDS Crystal Violet Methylrosanilinium Chloride
SDS, Safety Data Sheet
MSDS Sheet, Material Safety Data Sheet 29-Jun-21

Product Name & Other Names: Gentian Violet or Methylrosanilinium Chloride or Crystal violet or CI Basic Violet 3.
CAS No.: 548-62-9
EINECS EC Number: 208-953-6
Molecular Weight: 407.99
Chemical Formula: C25H30ClN3
Relevant uses and uses advised against (if any): Industrial Manufacturing.
Suppliers: As per letterhead.

GHS, Globally Harmonized System Classification in accordance with 29 CFR 1910
Classification according to Regulation (EC) No 1272/2008

Acute toxicity, Oral Category 4, H302
Serious eye damage Category 1, H318
Carcinogenicity Category 2, H351
Hazardous to the aquatic environment, long-term hazard Category 1, H410

Hazard statements:
H302: Harmful if swallowed.
H318: Causes serious eye damage.
H351: Suspected of causing cancer.
H410: Very toxic to aquatic life with long lasting effects.

Precautionary statements:
P201: Obtain special instructions before use.
P202: Do not handle until all safety precautions have been read and understood.
P264 Wash thoroughly after handling.
P270: Do not eat, drink or smoke when using this product.
P273: Avoid release to the environment.
P280: Wear protective gloves/protective clothing/eye protection/face protection.
P281: Use personal protective equipment as required.
P312: Call a POISON CENTER or doctor/physician if you feel unwell.
P330 : Rinse mouth
P301+P312: IF SWALLOWED: Call a POISON CENTER or doctor/ physician if you feel unwell.
P305+P351+P338: IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing.
P308+313: IF exposed or concerned: Get medical advice/attention.
P391: Collect spillage.
P405: Store locked up.
P501: Dispose of contents/container in accordance with local/regional/national/international regulation.

Product Name & Other Names: Gentian Violet or Methylrosanilinium Chloride or Crystal violet or CI Basic Violet 3.
CAS No.: 548-62-9
EINECS EC Number: 208-953-6

Inhalation: Allow the victim to rest in a well-ventilated area. Seek immediate medical attention.
Ingestion: Give large amounts of water to drink. Never give anything by mouth to an unconscious person. Get medical attention.
Skin Contact: Immediately flush skin with plenty of soap and water for at least 15 minutes. Remove contaminated clothing and shoes and wash before reuse. Get medical attention if irritation develops.
Eye Contact: Immediately flush eyes with running water for at least 15 minutes, keeping eyelids open. Cold water may be used. Get medical attention immediately.

Fire: As with most organic solids, fire is possible at elevated temperatures or by contact with an ignition source.
Explosion: Fine dust dispersed in air in sufficient concentrations, and in the presence of an ignition source is a potential dust explosion hazard.
Fire Extinguishing Media: Water spray, dry chemical, alcohol foam, or carbon dioxide.
Extinguishing Media Not recommended: Avoid using solid water jet as it may scatter the fire.
Special Information: In the event of a fire, wear full protective clothing and NIOSH-approved self-contained breathing apparatus with full face piece operated in the pressure demand or other positive pressure mode. At high temperatures under fire conditions, it may produce toxic or irritating fumes. Fire-extinguishing work is done from the windward and the suitable fire-extinguishing method according to the surrounding situation is used. Uninvolved persons should evacuate to a safe place.

Personal precautions, protective equipment, and emergency procedures: Avoid breathing dust/fumes/gas/mist/vapors/spray. Ensure adequate ventilation. Use individual protective equipment (waterproof boots, suitable protective clothing, safety glasses, etc.). Restrict unprotected personnel from the area. Prevent any contact with hot surfaces. Do not approach facing the wind. Do not touch the spilled material.
Environmental precautions: Do not let the product enter drains, soil, or water sources.
Methods and materials used for containment cleanup procedures and Storage:
Small Spill: Dilute with water and mop up or absorb with an inert dry material and place in an appropriate waste disposal container. Finish cleaning by spreading water on the contaminated surface and dispose of according to legal authority requirements.
Large Spill: Contain spilled material. Cover with an inert material and put the spilled material in an appropriate waste disposal. Finish cleaning by spreading water on the contaminated surface and dispose of according to legal authority requirements.

Precautions for safe handling: Apply according to good manufacturing and industrial hygiene practices. Ensure proper ventilation. Wash thoroughly after handling. Do not drink, eat, or smoke while handling. Avoid contact with skin, eyes, and clothing. Minimize dust generation. Avoid breathing dust/fumes/gas/mist/vapors/spray. Avoid contact with eyes, skin, and clothing. Keep container tightly closed. Avoid ingestion and inhalation. Use individual protective equipment (waterproof boots, suitable protective clothing, safety glasses, etc.). Prevent any contact with hot surfaces.
Conditions for safe storage, including any incompatibilities: Store in cool, dry, and ventilated area away from heat sources and protected from sunlight in tightly closed original container. Keep air contact to a minimum. Do not leave the material container open. Store protected from heat, sparks and ignition sources and incompatible materials. Avoid contact with skin and eyes. Avoid inhalation of dust/mist/vapor. Do not store with incompatible materials like strong oxidizers. Store away from sources of heat, moisture.

Airborne Exposure Limits: None established.
Ventilation System: A system of local and/or general exhaust is recommended to keep employee exposures as low as possible. Local exhaust ventilation is generally preferred because it can control the emissions of the contaminant at its source, preventing dispersion of it into the general work area. Please refer to the ACGIH document, Industrial Ventilation, A Manual of Recommended Practices, most recent edition, for details.
Personal Respirators (NIOSH Approved): For conditions of use where exposure to dust or mist is apparent and engineering controls are not feasible, a particulate respirator (NIOSH type N95 or better filters) may be worn. If oil particles (e.g., lubricants, cutting fluids, glycerin, etc.) are present, use a NIOSH type R or P filter. For emergencies or instances where the exposure levels are not known, use a full-face positive-pressure, air-supplied respirator. WARNING: Air-purifying respirators do not protect workers in oxygen-deficient atmospheres.
Skin Protection: Wear protective gloves and clean body-covering clothing.
Eye Protection: Use chemical safety goggles and/or full-face shield where dusting or splashing of solutions is possible. Maintain eye wash fountain and quick-drench facilities in work area.
Other Control Measures: Maintain good housekeeping in work area. Dust deposits on floors and other surfaces may pick up moisture and cause the surfaces to become slippery and present safety hazards. Handle in accordance with good industrial hygiene and safety practice. Wash hands after handling.

Appearance: Gentian Violet is dark green powder or greenish glistening pieces having a metallic luster.
Odor: Gentian Violet has slight characteristic odor.
Odor threshold: Not available.
pH: Not available.
Relative density: Not available.
Melting point/freezing point: 215C (419F).
Initial boiling point and boiling range: Not available.
Flash point: Not available.
Auto-ignition temperature: Not available.
Decomposition temperature: Not available.
Upper/lower flammability or explosive limits: Not available.
Vapor pressure: Not available.
Vapor density: Not available.
Evaporation rate: Not available.
Flammability (solid, gas): Not available.
Partition coefficient: n-octanol/water: Not available.
Solubility: Soluble in water.
Viscosity: Not available.
Molecular Weight: 407.99
Chemical Formula: C25H30ClN3

Stability: Stable under ordinary conditions of use and storage.
Hazardous Decomposition Products: When heated to decomposition it emits toxic fumes of nitrogen oxides and hydrogen chloride.
Hazardous Polymerization: Will not occur.
Incompatibilities: Strong oxidizers.
Conditions to Avoid: Incompatibles.

Acute toxicity:
LD50 Oral - Mouse - 96 mg/kg.
LD50 Oral - Rabbit - 150 mg/kg.
LD50 Intraperitoneal - Rat - 8,9 mg/kg.
LD50 Intraperitoneal - Mouse - 5,1 mg/kg.
LD50 Intraperitoneal - Rabbit - 5 mg/kg.
LD50 Intraduodenal - Rabbit - 160 mg/kg.
Investigated as a tumorigenic, mutagen, reproductive effector.
Carcinogenic Effects: Not a reported carcinogen by IARC NTP ACGIH OSHA.

Toxicity to daphnia and other aquatic invertebrates: EC50 - Daphnia magna (Water flea) - 0,35 mg/l - 48 h
Toxicity to algae: EC50 - Pseudokirchneriella subcapitata - 0,42 mg/l - 72 h
This substance/mixture contains no components considered to be either persistent, bioaccumulative and toxic (PBT), or very persistent and very bioaccumulative (vPvB) at levels of 0.1% or higher.

Whatever cannot be saved for recovery or recycling should be managed in an appropriate and approved waste disposal facility. Processing use or contamination of this product may change the waste management options. State and local disposal regulations may differ from federal disposal regulations. Dispose of container and unused material in accordance with legal requirements.

Land Transport DOT USA, TDG Canada & ADR/RID Europe:
UN/ID no UN3077
Hazard Class 9
Packing Group III
Proper shipping name Environmentally hazardous substance, solid, n.o.s. (Crystal Violet)
Sea Transport IMDG/IMO:
UN/ID no UN3077
Hazard Class 9
Packing Group III
Proper shipping name Environmentally hazardous substance, solid, n.o.s. (Crystal Violet)
Air Transport IATA/ICAO:
UN/ID no UN3077
Hazard Class 9
Packing Group III
Proper shipping name Environmentally hazardous substance, solid, n.o.s. (Crystal Violet).

USA:
SARA 311/312 Hazard Categories: Acute health hazard, Chronic Health Hazard. See section 2.
California Proposition 65: This product does not contain any Proposition 65 chemicals.

Gentian Violet Crystal Violet; Basic Violet 3; Methylrosanilinium Chloride Manufacturers, Suppliers, Exporters, Wholesellers:

Plot No. 2900/46&47 + 2900/163to167, GIDC, Ankleshwar, Dist. Bharuch, India
India, USA, UAE
TEL: (Office) 91-22-23774610, 91-22-23723564

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Our products are for industrial and laboratory use only. The user must test the material before use. We are not dispensing chemists or druggist and do not offer over the counter type (OTC) products for medical use by individuals.

Specifications, Properties, Uses, SDS of Gentian Violet Crystal Violet; Basic Violet 3; Methylrosanilinium Chloride USP Grade Manufacturer Supplier Exporter Wholesale & Small Packs, CAS Number 548-62-9.

Gentian Violet Crystal Violet; Basic Violet 3; Methylrosanilinium Chloride Manufacturers, Suppliers, Exporters, Wholesellers: